| 标题： | 小檗碱对严重腹腔感染时肠屏障功能障碍的防治作用 |
| 摘要： |
目的：探讨小檗碱对严重腹腔感染导致肠屏障功能障碍的防治作用。 方法：制备严重腹腔感染大鼠模型即盲肠结扎+穿孔模型（CLP模型），将90只SD大鼠随机分为感染组（CLP感染，不予治疗）、对照组（只剖腹而不行CLP术）和治疗组（盐酸小檗碱300mg/kg灌胃给药），各30只，观察各组肠道病理改变，进行血细菌培养，检测血清内毒素、二胺氧化酶（DAO）水平、D-乳酸含量评估其肠屏障功能；同时检测血清C反应蛋白（CRP）、白介素2（IL-2）、肿瘤坏死因子α（TNF-α）水平等炎症相关因子，了解炎症反应情况。 结果：治疗组肠黏膜损伤明显减轻；在术后第1、2、4天检测点上，治疗组血清内毒素、DAO及D-乳酸水平明显低于感染组（P﹤0.01）。同时治疗组TNF-α、血清CRP及IL-2水平明显低于感染组（P﹤0.01）。 结论：严重腹腔感染时过度炎症反应可导致肠屏障功能障碍，小檗碱通过降低炎症反应而实现对肠屏障功能的保护。 |
| 英文摘要： | Objective：To discuss the preventive and treatment effect of Berbrine on intestinal barrier dysfunction in rats with severe abdominal infection. Methods：Ninety model rats of severe abdominal infection were prepared for cecal ligation and puncture (CLP). They were randomly divided into three groups: the severe abdominal infection group (prepared by CLP), the control group (laparotomy only) and the Berberine treatment group. The changes of intestinal pathology in each group were observed, blood culture and plasma endotoxin, DAO, D-lactate were taken at different times after the operation. Meanwhile, the inflammatory factor, such as TNF-α, CRP and IL-2 were tested for the inflammatory level. Results：The damage of intestinal mucosa in CLP group was more severe than that in Berberine treatment group, and plasma endotoxin, DAO and D-lactate level, TNF-α, CRP and IL-2 level, were higher in CLP group, which meant less inflammatory reaction after Berbrine treatment. Conclusion：Excessive inflammatory reaction in rats with severe abdominal infection results in intestinal barrier dysfunction, and Berbrine protects the intestinal barrier function by reducing the inflammatory damage. |
| 作者： |
罗育其；吴承堂；于海涛；董栋 |
| 作者单位： | 广州，南沙中心医院普通外科（罗育其、于海涛、董栋）；南方医科大学附属南方医院普通外科（吴承堂） |
| 期刊： | 中华普通外科学文献(电子版) |
| 年.卷(期):页码 | 2014 .8（3）：191-195 |
| 中图分类号： | |
| 文章编号： | |
| 引用格式： | [1]罗育其；吴承堂；于海涛；董栋.小檗碱对严重腹腔感染时肠屏障功能障碍的防治作用[J/CD].中华普通外科学文献（电子版）,2014,8(3):191-195. |
| 关键词： | 小檗碱 肠屏障功能 严重腹腔感染 炎症反应 |
| 英文关键词： | Berberine；Intestinal barrier function；Severe abdominal infection；Inflammatory reaction |
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