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中华普通外科学文献简介:中华普通外科学文献简介,中华普通外科学文献简介[详细]

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主管:
中华人民共和国国家卫生健康委员会
主办:
中华医学会
承办:
中山大学附属第一医院
中山大学附属中山医院
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中华医学电子音像出版社有限责任公司
总编辑: 王深明
编辑部主任: 汪谦
刊 期: 双月刊
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国际刊号: 1674-0793
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首页 > 期刊列表 > 血清微小RNA34a联合癌胚抗原、糖链抗原72-4预测胃癌根治术后早期复发转移
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标题: 血清微小RNA34a联合癌胚抗原、糖链抗原72-4预测胃癌根治术后早期复发转移
摘要:

目的:探讨血清微小RNA34a(miR-34a)联合癌胚抗原(CEA)、糖链抗原72-4(CA72-4)检测在胃癌根治术后早期复发转移的预测价值。方法:选择2014年6月至2015年8月哈尔滨医科大学附属第一医院收治的胃癌患者126例,根据胃癌根治术后随访结局分为复发转移组58例,未复发转移组68例。荧光定量PCR检测血清miR-34a表达水平,电化学发光免疫分析法检测血清CEA、CA72-4水平。单因素分析miR-34a、CEA、CA72-4水平与患者临床病理特征的关系,Cox多因素分析影响胃癌根治术后复发转移的独立危险因素,ROC曲线评估各指标对胃癌根治术后早期复发转移的预测价值。结果:与未复发转移组相比,复发转移组患者血清miR-34a表达水平更低(t=6.142,P<0.001),血清CEA和CA72-4水平更高(t=17.985、21.415,均P<0.001)。血清miR-34a(P=0.013)、CEA(P=0.013)、CA72-4(P=0.011)、淋巴结转移(P=0.048)是影响胃癌患者复发转移的独立危险因素。三种指标单独检测比较,CA72-4敏感度最高(94.8%),miR-34a最低(70.7%);miR-34a的特异度最高(94.1%),CA72-4最低(73.5%)。血清miR-34a、CEA、CA72-4联合预测胃癌术后早期复发转移的曲线下面积为0.954(95%CI: 0.923~0.985),敏感度为87.9%,特异度为89.7%。结论:血清miR-34a在胃癌根治术后早期复发转移患者中表达下调,而血清CEA、CA72-4水平升高,三者联合检测可为预测胃癌根治术后早期复发转移提供重要的参考。

英文摘要: Objective:To explore the detective value of serum micro RNA34a (miR-34a) combined with carcinoembryonic antigen (CEA) and carbohydrate antigen 72-4 (CA72-4) in predicting early recurrence and metastasis of gastric cancer after radical gastrectomy. Methods:A total of one hundred and twenty-six patients with gastric cancer who underwent radical gastrectomy in the First Affiliated Hospital of Harbin Medical University from June 2014 to August 2015 were selected and divided into recurrence and metastasis group (58 cases, metastatic group) and non-recurrence and metastasis group (68 cases, non-metastatic group). The expression levels of serum miR-34a were detected by real-time fluorescent quantitative PCR, serum CEA and CA72-4 levels were detected by electro-chemiluminescence immunoassay. Univariate analysis of the association was carried out between the levels of miR-34a, CEA and CA72-4 and the clinic pathological characteristics of patients, while Cox regression analysis was carried out of the independent risk factors affecting early recurrence and metastasis of gastric cancer after radical gastrectomy. The predictive value of early recurrence and metastasis of gastric cancer after radical gastrectomy was analyzed by ROC curve. Results:Compared to non-metastatic group, the expression level of serum miR-34a in metastatic group after radical gastrectomy was significantly lower (t=6.142, P<0.001), and serum CEA and CA72-4 levels were significantly higher (t=17.985, 21.415, both P<0.001). SerummiR-34a (P=0.013), CEA (P=0.013), CA72-4 (P=0.011), and lymph node metastasis (P=0.048) were independent risk factors for recurrence and metastasis of gastric cancer after radical gastrectomy. Individual detection of three indicators showed that the sensitivity of CA72-4 was the highest (94.8%) and that of miR-34a was the lowest (70.7%); the specificity of miR-34a was the highest (94.1%) and that of CA72-4 was the lowest (73.5%). The AUC of serum miR-34a, CEA, and CA72-4 combined detection in predicting early recurrence and metastasis after radical gastrectomy was 0.954 (95%CI: 0.923-0.985), the sensitivity was 87.9%, and the specificity was 89.7%. Conclusion:The expression of serum miR-34a in patients with early recurrence and metastasis of gastric cancer after radical gastrectomy is down-regulated, while the levels of serum CEA and CA72-4 are increased, and combined detection of the three indicators can provide important reference for screening early recurrence and metastasis of gastric cancer after radical gastrectomy.
作者:

许丹;杨幼林

作者单位: 哈尔滨医科大学附属第一医院消化内科
期刊: 中华普通外科学文献(电子版)
年.卷(期):页码 2019 .13(6):463-467
中图分类号:
文章编号:
引用格式: [1]许丹;杨幼林.血清微小RNA34a联合癌胚抗原、糖链抗原72-4预测胃癌根治术后早期复发转移[J/CD].中华普通外科学文献(电子版),2019,13(6):463-467 .
关键词: 微RNAs;胃肿瘤;胃切除术;复发转移;癌胚抗原;糖链抗原72-4
英文关键词: MicroRNAs;Stomach neoplasms;Gastrectomy;Recurrence;Carcinoembryonic antigen;Carbohydrate antigen 72-4

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